長坂和明 | | 新潟医療福祉大学

リハビリテーション学部

Department of Physical Therapy 理学療法学科

教員紹介

長坂和明写真

リハビリテーション学部／理学療法学科
Faculty of Rehabilitation／Department of Physical Therapy

長坂和明 Kazuaki Nagasaka

講師

プロフィール

担当科目	理学療法基礎評価学Ⅱ、神経筋機能解析学、神経筋疾患治療学、痛み学
専門分野	神経科学、脳計測科学
キーワード	脳卒中後疼痛、神経可塑性、動物実験
学位・称号／取得機関／取得年	博士（神経科学）／筑波大学／2018年修士（神経科学）／筑波大学／2015年
資格	理学療法士
過去の経歴	産業技術総合研究所　博士研究員、新潟医療福祉大学　運動機能医科学研究所　研究員を経て現職
所属団体・学会等	日本理学療法士協会、日本疼痛学会、日本神経科学学会、日本生理学会、脳機能とリハビリテーション研究会

外部リンク

業績等

研究領域	痛みの脳内情報処理、システム神経生理学、脳卒中
研究紹介	脳卒中の後には様々な後遺症が出現しますが、その中でも‟病的な痛み”は病態メカニズムが不明で、確立された治療法がなく、困っている患者さんが多いのが現状です。私の研究では、動物モデルを用いた細胞レベルの実験から、近年では健常人や患者さんを対象とした実験的データも取り入れて、病態の統合的な解釈を目指しています。
受賞歴	1. 2021年第25回日本基礎理学療法学会学術大会　奨励賞 2. 2018年筑波大学人間総合科学研究科　研究科長賞 3. 2018年第52回日本理学療法学術大会　奨励賞 4. 2017年第1回ヒト脳機能イメージング研究会　奨励賞 5. 2015年第22回脳機能とリハビリテーション研究会学術集会　基礎研究部門最優秀発表賞
研究論文（英文）	【筆頭国際誌】 1. Nagasaka K, Takashima I, Matsuda K, Higo N. Pharmacological inactivation of the primate posterior insular/secondary somatosensory cortices attenuates thermal hyperalgesia, European Journal of Pain, 2022 26(8):1723-1731 2. Nagasaka K, Otsuru N, Sato R, Watanabe H, Sakurai N, Ohno K, Kodama N, Onishi H. Cortical signature related to psychometric properties of pain vigilance in healthy individuals: a voxel-based morphometric study, Neuroscience letters, 2022 772:136445, 3. Nagasaka K, Nemoto K, Takashima I, Bando D, Matsuda K, Higo N. Structural Plastic Changes of Cortical Gray Matter Revealed by Voxel-Based Morphometry and Histological Analyses in a Monkey Model of Central Post-Stroke Pain, Cerebral Cortex, 2021 31(10):4439-4449, 4. Nagasaka K, Takashima I, Matsuda K, Higo N. Brain activity changes in a monkey model of central post-stroke pain, Experimental Neurology, 2020 323,113096, 5. Nagasaka K, Takashima I, Matsuda K, Higo N. Late-onset hypersensitivity after a lesion in the ventral posterolateral nucleus of the thalamus: A macaque model of central post-stroke pain, Scientific Reports, 2017 7:10316, 6. Nagasaka K, Yamanaka K, Ogawa S, Takamatsu H, Higo N. Brain activity changes in a macaque model of oxaliplatin-induced neuropathic cold hypersensitivity, Scientific Reports, 2017 7:4305, 7. Nagasaka K, Watanabe Y, Takashima I. Topographical projections from the nucleus basalis magnocellularis (Meynert) to the frontal cortex: A voltage-sensitive dye imaging study in rats, Brain Stimulation, 2017 10(5):977-980, 【共著国際誌/国内誌】 1. Watanabe Y, Dezawa S, Takei H, Nagasaka K, Takashima I. Hippocampal-prefrontal long-term potentiation-like plasticity with transcranial direct current stimulation in rats, Neurobiology of Learning and Memory, 2023.3 201:107750 2. Sakurai N, Nagasaka K, Takahashi S, Kasai S, Onishi H, Kodama N. Brain function effects of autonomous sensory meridian response (ASMR) video viewing, Frontiers in neuroscience, 2023 17:1025745 3. Onishi H, Nagasaka K, Yokota H, Kojima S, Ohno K, Sakurai N, Kodama N, Sato D, Otsuru N. Association between somatosensory sensitivity and regional gray matter volume in healthy young volunteers: a voxel-based morphometry study, Cerebral cortex, 2022 33(5):2001-2010 4. Saito K, Otsuru N, Inukai Y, Kojima S, Miyaguchi S, Nagasaka K, Onishi H. Effect of Transcranial Electrical Stimulation over the Posterior Parietal Cortex on Tactile Spatial Discrimination Performance, Neuroscience, 2022 494:94-103 5. Kobayashi R, Sakurai N, Nagasaka K, Kasai S, Kodama N. Relationship between Tactile Sensation, Motor Activity, and Differential Brain Activity in Young Individuals, Brain Sciences, 2022 12(7):924, 6. Pham M, Saito K, Miyaguchi S, Watanabe H, Ikarashi H, Nagasaka K, Yokota H, Kojima S, Inukai Y, Otsuru N, Onishi H. Changes in excitability and GABAergic neuronal activity of the primary somatosensory cortex after motor learning, Frontiers in Neuroscience, 2022 16:794173 7. Matsuda K, Nagasaka K, Kato J, Takashima I, Higo N. Structural plasticity of motor cortices assessed by voxel-based morphometry and immunohistochemical analysis following internal capsular infarcts in macaque monkeys, Cerebral Cortex Communications, 2022 3(4):1-14 8. Ikarashi H, Otsuru N, Yokota H, Nagasaka K, Igarashi K, Miyaguchi S, Onishi H, Influence of Catechol-O-Methyltransferase Gene Polymorphism on the Correlation between Alexithymia and Hypervigilance to Pain, International Journal of Environmental Research and Public Health, 2021 18(24):13265-13265, 9. Sakurai N, Ohno K, Kasai S, Nagasaka K, Onishi H, Kodama N, Induction of Relaxation by Autonomous Sensory Meridian Response, Frontiers in Behavioral Neuroscience, 2021 15:761621, 10. Watanabe H, Kojima S, Nagasaka K, Ohno K, Sakurai N, Kodama N, Otsuru N, Onishi H, Gray Matter Volume Variability in Young Healthy Adults: Influence of Gender Difference and Brain-Derived Neurotrophic Factor Genotype, Cerebral Cortex, 2021 32(12):2635-2643 11. Kojima S, Otsuru N, Miyaguchi S, Yokota H, Nagasaka K, Saito K, Inukai Y, Shirozu H, Onishi H, The intervention of mechanical tactile stimulation modulates somatosensory evoked magnetic fields and cortical oscillations, European Journal of Neuroscience, 2021 53(10):3433-3446, 12. Yokota H, Otsuru N, Saito K, Kojima S, Miyaguchi S, Inukai Y, Nagasaka K, Onishi H, Region-Specific Effects of 10-Hz Transcranial Alternate Current Stimulation Over the Left Posterior Parietal Cortex and Primary Somatosensory Area on Tactile Two-Point Discrimination Threshold, Frontiers in Neuroscience, 2021 15:576526, 13. Dezawa S, Nagasaka K, Watanabe Y, Takashima I. Lesions of the nucleus basalis magnocellularis (Meynert) induce enhanced somatosensory responses and tactile hypersensitivity in rats. Experimental Neurology, 2020 335:113493, 14. Gotoh M, Nagasaka K, Nakata M, Takashima I, Yamamoto S. Brain Temperature Alters Contributions of Excitatory and Inhibitory Inputs to Evoked Field Potentials in the Rat Frontal Cortex. Frontiers in Cellular Neuroscience, 2020 14:593027, 15. Inukai Y, Otsuru N, Saito K, Miyaguchi S, Kojima S, Yokota H, Nagasaka K, Onishi H. The after-effect of noisy galvanic vestibular stimulation on postural control in young people: A randomized controlled trial. Neuroscience letters, 2020 729:135009, 16. Nakata M, Nagasaka K, Shimoda M, Takashima I, Yamamoto S. Focal brain lesions induced with ultraviolet irradiation. Scientific Reports, 2018 7968
研究論文（和文）	1. Ota T, Yamamoto T, Sako R, Fujii A, Nagasaka K, Hondera T, Ozasa Y, Successive evaluation of clinical findings and brain images in left thalamic hemorrhage with a previous right putaminal hemorrhage, Journal of Rehabilitation Neurosciences, 2020 20:42-48,
総説・解説	1. 長坂和明，肥後範行．サルモデルを用いた異常な痛みを引き起こす神経可塑性の解明，日本基礎理学療法学雑誌， 2019 22(1) 18-24, 2. 肥後範行，長坂和明．マカクサルを用いた脳卒中後疼痛動物モデルの確立とメカニズム解明に向けた挑戦， Pain Research, 2018 33 275-281,
科学研究費	1. (2023-2026年度) 基盤研究（B）脳卒中後疼痛の発症を予測する神経活動バイオマーカーの確立と新たな治療戦略の創出 2. (2020-2022年度) 若手研究島皮質の活動をリモート制御する大脳皮質電気刺激法の確立 3. (2017-2019年度) その他脳損傷後に痛みを誘発する神経ネットワークの大規模改変：サルモデルによる統合的解析
学会等研究発表	1. 長坂和明，高島一郎，松田圭司，肥後範行後部島皮質・二次体性感覚野の薬理学的抑制は温熱刺激に対する痛覚過敏を減弱させる－脳卒中後疼痛サルモデルを対象とした検証－第28回脳機能とリハビリテーション研究会学術集会 2022/9/1-2022/9/23 2. 大槻智史，長坂和明，大鶴直史，大西秀明扁桃体外側基底核の電気刺激によって誘発される後部島皮質の神経活動応答‐脳卒中後疼痛モデルラットを対象とした計測第28回脳機能とリハビリテーション研究会学術集会 2022/9/1-2022/9/23 3. 松田滉平，長坂和明，加藤隼平，高島一郎，肥後範行マカクサル内包梗塞後における脳灰白質の可塑的構造変化第28回脳機能とリハビリテーション研究会学術集会 2022/9/1-2022/9/23 4. 五十嵐眸実，大鶴直史，長坂和明，宮口翔太，Jose Gomez-Tames，平田晃正，大西秀明電界シミュレーションを用いた経頭蓋交流電流による鎮痛効果の検討第28回脳機能とリハビリテーション研究会学術集会 2022/9/1-2022/9/23 5. 鈴木孝昇, 長坂和明, 大槻智史, 大鶴直史, 大西秀明 The effects of tonic locus coeruleus output on forepaw-evoked response of the primary somatosensory cortex in anesthetized rats 第45回日本神経科学大会 2022/6/30-7/3 6. 鈴木孝昇, 長坂和明, 大槻智史, 大鶴直史, 大西秀明青斑核への持続的な1.0Hz電気刺激は一次体性感覚野の活動を増大させる一麻酔下動物実験による検証一第22回新潟医療福祉学会学術集会 2022/10/29 7. 五十嵐眸実，大鶴直史，長坂和明，宮口翔太，Jose Gomez-Tames，平田晃正，大西秀明背外側前頭前野に対する経頭蓋交流電流刺激による鎮痛効果-電界シミュレーションによる検討- 第27回日本基礎理学療法学会学術大会 2022/10/1-10/2 8. 大槻智史，長坂和明，大鶴直史，大西秀明 Neural response in the posterior insular cortex evoked by stimulation of the basolateral amygdala in central post-stroke pain model rats 第100回日本生理学会 2023/3/14
学会・職能団体の委員	1. 神経理学療法学　査読委員 2. 理学療法学　査読委員 3. 第28回脳機能とリハビリテーション研究会学術集会　大会実行委員長 4. 脳機能とリハビリテーション研究会理事 5. Journal of Rehabilitation Neurosciences Editor 6. 理学療法ガイドライン・システマティックレビュー ”神経難病” 班員
公開講座・講演会等	1. 2022年度第40回脳機能とリハビリテーション研究会勉強会視床感覚核の基礎研究と視床痛講師脳機能とリハビリテーション研究会理学療法士，作業療法士，他 Web開催 2022/5/25 2. 2022年度脳卒中リハビリテーション研究センター【脳卒中リハビリテーションにおける画像評価の基礎と臨床応用】脳卒中リハビリテーションに必要な画像評価の基礎知識講師脳卒中リハビリテーション研究センター理学療法士，作業療法士，他 Web開催 2022/7/9 3. 2022年度みとスポーツ医科学研究会オンラインセミナー脳と痛み基礎研究によって根拠に基づく体系化された評価・治療技術確立に挑む講師みとスポーツ医科学研究会理学療法士，作業療法士，他 Web開催 2022/8/24 4. 2022年度第20回日本神経理学療法学会学術大会　シンポジウム脳卒中後疼痛の発症メカニズムと神経モジュレーションによる除痛機序の解明－動物モデルを用いた研究－シンポジスト日本神経理学療法学会理学療法士，作業療法士，他大阪市，大阪国際会議場 2022/10/15-10/16 5. 2022年度日本神経理学療法学会　第27回サテライトカンファレンス中枢性疼痛の病態メカニズムー脳卒中後疼痛を中心に－講師日本神経理学療法学会理学療法士，作業療法士，他北葛城郡広陵町，畿央大学 2023/2/11 6. 2022年度人間情報インタラクション研究部門シンポジウム 2022 ～脳の変化～脳卒中後の病的な痛みのメカニズム解明とその克服に向けて講師産業技術総合研究所研究者他 Web開催 2023/2/18
学内委員会活動	1. 高等教育推進センター（委員） 2. 運動機能医科学研究所（研究員）
知的財産権	1. 2022年度特許：視床痛を有する非ヒト霊長類モデル動物肥後範行, 高島一郎, 松田圭司, 長坂和明, 猪股伸一 2014.8.27 6481166 2. 2022年度特許：ドーパミン検出用蛍光物質鈴木祥夫, 山本慎也, 長坂和明, 高島一郎 2015.11.30 6685546 3. 2022年度特許：脳表を局所的に損傷させる方法仲田真理子, 長坂和明, 下田将之, 高島一郎, 山本慎也 2017.11.1 7071728

Profile

Research Areas	System neuroscience, Chronic pain
Research Introduction	I am interested in neural plasticity after lesions of the central nervous system. My main approaches to elucidate this are brain activity measurements (imaging and electrophysiology) and electrical stimulation interventions in animals and humans.
Papers	【筆頭国際誌】 1. Nagasaka K, Takashima I, Matsuda K, Higo N. Pharmacological inactivation of the primate posterior insular/secondary somatosensory cortices attenuates thermal hyperalgesia, European Journal of Pain, 2022 26(8):1723-1731 2. Nagasaka K, Otsuru N, Sato R, Watanabe H, Sakurai N, Ohno K, Kodama N, Onishi H. Cortical signature related to psychometric properties of pain vigilance in healthy individuals: a voxel-based morphometric study, Neuroscience letters, 2022 772:136445, 3. Nagasaka K, Nemoto K, Takashima I, Bando D, Matsuda K, Higo N. Structural Plastic Changes of Cortical Gray Matter Revealed by Voxel-Based Morphometry and Histological Analyses in a Monkey Model of Central Post-Stroke Pain, Cerebral Cortex, 2021 31(10):4439-4449, 4. Nagasaka K, Takashima I, Matsuda K, Higo N. Brain activity changes in a monkey model of central post-stroke pain, Experimental Neurology, 2020 323,113096, 5. Nagasaka K, Takashima I, Matsuda K, Higo N. Late-onset hypersensitivity after a lesion in the ventral posterolateral nucleus of the thalamus: A macaque model of central post-stroke pain, Scientific Reports, 2017 7:10316, 6. Nagasaka K, Yamanaka K, Ogawa S, Takamatsu H, Higo N. Brain activity changes in a macaque model of oxaliplatin-induced neuropathic cold hypersensitivity, Scientific Reports, 2017 7:4305, 7. Nagasaka K, Watanabe Y, Takashima I. Topographical projections from the nucleus basalis magnocellularis (Meynert) to the frontal cortex: A voltage-sensitive dye imaging study in rats, Brain Stimulation, 2017 10(5):977-980, 【共著国際誌/国内誌】 1. Watanabe Y, Dezawa S, Takei H, Nagasaka K, Takashima I. Hippocampal-prefrontal long-term potentiation-like plasticity with transcranial direct current stimulation in rats, Neurobiology of Learning and Memory, 2023.3 201:107750 2. Sakurai N, Nagasaka K, Takahashi S, Kasai S, Onishi H, Kodama N. Brain function effects of autonomous sensory meridian response (ASMR) video viewing, Frontiers in neuroscience, 2023 17:1025745 3. Onishi H, Nagasaka K, Yokota H, Kojima S, Ohno K, Sakurai N, Kodama N, Sato D, Otsuru N. Association between somatosensory sensitivity and regional gray matter volume in healthy young volunteers: a voxel-based morphometry study, Cerebral cortex, 2022 33(5):2001-2010 4. Saito K, Otsuru N, Inukai Y, Kojima S, Miyaguchi S, Nagasaka K, Onishi H. Effect of Transcranial Electrical Stimulation over the Posterior Parietal Cortex on Tactile Spatial Discrimination Performance, Neuroscience, 2022 494:94-103 5. Kobayashi R, Sakurai N, Nagasaka K, Kasai S, Kodama N. Relationship between Tactile Sensation, Motor Activity, and Differential Brain Activity in Young Individuals, Brain Sciences, 2022 12(7):924, 6. Pham M, Saito K, Miyaguchi S, Watanabe H, Ikarashi H, Nagasaka K, Yokota H, Kojima S, Inukai Y, Otsuru N, Onishi H. Changes in excitability and GABAergic neuronal activity of the primary somatosensory cortex after motor learning, Frontiers in Neuroscience, 2022 16:794173 7. Matsuda K, Nagasaka K, Kato J, Takashima I, Higo N. Structural plasticity of motor cortices assessed by voxel-based morphometry and immunohistochemical analysis following internal capsular infarcts in macaque monkeys, Cerebral Cortex Communications, 2022 3(4):1-14 8. Ikarashi H, Otsuru N, Yokota H, Nagasaka K, Igarashi K, Miyaguchi S, Onishi H, Influence of Catechol-O-Methyltransferase Gene Polymorphism on the Correlation between Alexithymia and Hypervigilance to Pain, International Journal of Environmental Research and Public Health, 2021 18(24):13265-13265, 9. Sakurai N, Ohno K, Kasai S, Nagasaka K, Onishi H, Kodama N, Induction of Relaxation by Autonomous Sensory Meridian Response, Frontiers in Behavioral Neuroscience, 2021 15:761621, 10. Watanabe H, Kojima S, Nagasaka K, Ohno K, Sakurai N, Kodama N, Otsuru N, Onishi H, Gray Matter Volume Variability in Young Healthy Adults: Influence of Gender Difference and Brain-Derived Neurotrophic Factor Genotype, Cerebral Cortex, 2021 32(12):2635-2643 11. Kojima S, Otsuru N, Miyaguchi S, Yokota H, Nagasaka K, Saito K, Inukai Y, Shirozu H, Onishi H, The intervention of mechanical tactile stimulation modulates somatosensory evoked magnetic fields and cortical oscillations, European Journal of Neuroscience, 2021 53(10):3433-3446, 12. Yokota H, Otsuru N, Saito K, Kojima S, Miyaguchi S, Inukai Y, Nagasaka K, Onishi H, Region-Specific Effects of 10-Hz Transcranial Alternate Current Stimulation Over the Left Posterior Parietal Cortex and Primary Somatosensory Area on Tactile Two-Point Discrimination Threshold, Frontiers in Neuroscience, 2021 15:576526, 13. Dezawa S, Nagasaka K, Watanabe Y, Takashima I. Lesions of the nucleus basalis magnocellularis (Meynert) induce enhanced somatosensory responses and tactile hypersensitivity in rats. Experimental Neurology, 2020 335:113493, 14. Gotoh M, Nagasaka K, Nakata M, Takashima I, Yamamoto S. Brain Temperature Alters Contributions of Excitatory and Inhibitory Inputs to Evoked Field Potentials in the Rat Frontal Cortex. Frontiers in Cellular Neuroscience, 2020 14:593027, 15. Inukai Y, Otsuru N, Saito K, Miyaguchi S, Kojima S, Yokota H, Nagasaka K, Onishi H. The after-effect of noisy galvanic vestibular stimulation on postural control in young people: A randomized controlled trial. Neuroscience letters, 2020 729:135009, 16. Nakata M, Nagasaka K, Shimoda M, Takashima I, Yamamoto S. Focal brain lesions induced with ultraviolet irradiation. Scientific Reports, 2018 7968 【First/Corresponding author】 1. Nagasaka K, Otsuru N, Sato R, Watanabe H, Sakurai N, Ohno K, Kodama N, Onishi H. Cortical signature related to psychometric properties of pain vigilance in healthy individuals: a voxel-based morphometric study. Neuroscience letters, 772:136445, 2022 2. Nagasaka K, Nemoto K, Takashima I, Bando D, Matsuda K, Higo N. Structural Plastic Changes of Cortical Gray Matter Revealed by Voxel-Based Morphometry and Histological Analyses in a Monkey Model of Central Post-Stroke Pain. Cerebral Cortex, 31(10):4439-4449, 2021 3. Nagasaka K, Takashima I, Matsuda K, Higo N. Brain activity changes in a monkey model of central post-stroke pain. Experimental Neurology, 323,113096, 2020 4. Nagasaka K, Takashima I, Matsuda K, Higo N. Late-onset hypersensitivity after a lesion in the ventral posterolateral nucleus of the thalamus: A macaque model of central post-stroke pain. Scientific Reports, 7:10316, 2017 5. Nagasaka K, Yamanaka K, Ogawa S, Takamatsu H, Higo N. Brain activity changes in a macaque model of oxaliplatin-induced neuropathic cold hypersensitivity. Scientific Reports, 7:4305, 2017 6. Nagasaka K, Watanabe Y, Takashima I. Topographical projections from the nucleus basalis magnocellularis (Meynert) to the frontal cortex: A voltage-sensitive dye imaging study in rats. Brain Stimulation, 10(5):977-980, 2017 【Co-author】 1. Ikarashi H, Otsuru N, Yokota H, Nagasaka K, Igarashi K, Miyaguchi S, Onishi H,Influence of Catechol-O-Methyltransferase Gene Polymorphism on the Correlation between Alexithymia and Hypervigilance to Pain, International Journal of Environmental Research and Public Health, 18(24):13265-13265, 2021 2. Sakurai N, Ohno K, Kasai S, Nagasaka K, Onishi H, Kodama N, Induction of Relaxation by Autonomous Sensory Meridian Response, Frontiers in Behavioral Neuroscience, 15:761621, 2021 3. Watanabe H, Kojima S, Nagasaka K, Ohno K, Sakurai N, Kodama N, Otsuru N, Onishi H, Gray Matter Volume Variability in Young Healthy Adults: Influence of Gender Difference and Brain-Derived Neurotrophic Factor Genotype, Cerebral Cortex, in press 4. Kojima S, Otsuru N, Miyaguchi S, Yokota H, Nagasaka K, Saito K, Inukai Y, Shirozu H, Onishi H, The intervention of mechanical tactile stimulation modulates somatosensory evoked magnetic fields and cortical oscillations, European Journal of Neuroscience, 53(10):3433-3446, 2021 5. Yokota H, Otsuru N, Saito K, Kojima S, Miyaguchi S, Inukai Y, Nagasaka K, Onishi H, Region-Specific Effects of 10-Hz Transcranial Alternate Current Stimulation Over the Left Posterior Parietal Cortex and Primary Somatosensory Area on Tactile Two-Point Discrimination Threshold, Frontiers in Neuroscience, 15:576526, 2021 6. Dezawa S, Nagasaka K, Watanabe Y, Takashima I. Lesions of the nucleus basalis magnocellularis (Meynert) induce enhanced somatosensory responses and tactile hypersensitivity in rats. Experimental Neurology, 335:113493, 2020 7. Gotoh M, Nagasaka K, Nakata M, Takashima I, Yamamoto S. Brain Temperature Alters Contributions of Excitatory and Inhibitory Inputs to Evoked Field Potentials in the Rat Frontal Cortex. Frontiers in Cellular Neuroscience, 14:593027, 2020 8. Ota T, Yamamoto T, Sako R, Fujii A, Nagasaka K, Hondera T, Ozasa Y, Successive evaluation of clinical findings and brain images in left thalamic hemorrhage with a previous right putaminal hemorrhage, Journal of Rehabilitation Neurosciences, 20:42-48, 2020 9. Inukai Y, Otsuru N, Saito K, Miyaguchi S, Kojima S, Yokota H, Nagasaka K, Onishi H. The after-effect of noisy galvanic vestibular stimulation on postural control in young people: A randomized controlled trial. Neuroscience letters, 729:135009, 2020